New Delhi: Physicists have now found a method to quickly identify single antibiotic-resistant bacteria cells that are the agents of tuberculosis. The new method helps find the bacteria and evaluate their resistance to antibiotics without damaging the biological material. The study was carried out at the Immanuel Kant Baltic State University and the results of the first trial of the method were published in Data in Brief.
A team of researchers from Immanuel Kant Baltic Federal University together with their colleagues from Saint-Petersburg State Research Institute of Phthisiopulmonology and Saint-Petersburg State University suggested using Raman scattering spectrography to quickly analyse bacterial cells.
This method helps identify the composition and structure of the studied material based on the scattering of laser radiation with certain wavelength by its sample. Spectroscopy is a non-invasive method, i.e. the material under study is not subject to any mechanical impact or destruction.
To obtain information about the structure of the cells belonging to different strains, the scientists pointed the laser beam at different bacteria during the spectroscopy procedure. The cells of different strains appeared to scatter the light differently because resistance to antibiotics occurs, among other things, due to changes in the composition of bacterial cell wall components.
Spectroscopy helped identify differences in the cell walls of drug-resistance and drug-sensitive bacteria.